那不勒斯腓特烈二世大学（意大利语：Università degli Studi di Napoli Federico II；英语：University of Naples Federico II）是意大利历史最悠久的国立大学，创建于1224年，位于南部城市那不勒斯。作为世界上连续办学时间最长的学术机构之一，该校以神圣罗马帝国皇帝腓特烈二世的名字命名，至今仍保留着深厚的学术传统与创新活力。

University of Naples Federico II (Italian: Università degli Studi di Napoli Federico II) is the oldest state university in Italy, founded in 1224 and located in the southern city of Naples. As one of the world's longest continuously operating academic institutions, it is named after Holy Roman Emperor Frederick II and retains a profound academic tradition alongside innovative vitality to this day.

该校设有13个学院，涵盖农业、工程、法律、医学、文学、科学等多个领域，提供88个本科专业和83个研究生专业。在2024年QS世界大学排名中位列第335位，农业工程、土木工程、食品科学等学科跻身全球前100，其中航空航天工程专业在软科排名中位列世界第34位。现有约8万名学生，校园分布于那不勒斯市区，部分建筑为历史悠久的贵族宫殿改造而成。

The university comprises 13 faculties spanning agriculture, engineering, law, medicine, humanities, and sciences, offering 88 undergraduate and 83 graduate programs. Ranked 335th in the 2024 QS World University Rankings, its strengths include Agricultural Engineering, Civil Engineering, and Food Science (all top 100 globally), with Aerospace Engineering ranked 34th worldwide by ShanghaiRanking. Hosting approximately 80,000 students, its campus spans Naples' urban area, with some buildings converted from historic aristocratic palaces.